Mounting Media
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Kaiser’s Glycerin Jelly
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| Ingredients | ||
| distilled water |
52 ml
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| gelatin |
8 gm
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| glycerin |
50 ml
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| thymol (to prevent mold) |
a crystal
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Directions |
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Heat to 75°C and stir until dissolved. Do not heat above this temperature. Excellent mounting medium for wholemounts. Specimen is taken from 70% ETOH or water, and put through a graded series of glycerin solutions (20% glycerin, 50% glycerin, 100% glycerin; series can be of glycerin mixed with 70% ETOH or water). When the specimen is translucent (cleared) from having been in 100% glycerin for a sufficient amount of time, it is mounted on a slide using glycerin jelly that has been warmed until it is liquid. A coverslip is placed on top of the warmed glycerin jelly. To warm the glycerin jelly, use a water-bath, or place the container in a beaker of water on a warming plate set on low heat. Do not overheat. Once the glycerin jelly on the slide has become firm, the coverslip is ringed with a sealing agent such as finger-nail polish. |
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